Download Atomic Force Microscopy in Liquid: Biological Applications by Arturo M. Baró, Ronald G. Reifenberger PDF

By Arturo M. Baró, Ronald G. Reifenberger

This primary ebook to target all rules and features of AFM in liquid section is completely based, making it easy-to-follow for non-AFM experts. while, it truly is an exceptional advent for researchers wishing to take advantage of this significant method for comparing organic fabric and organic applications.
From the contents:
* AFM: simple concept
* Dynamic modes in liquids
* strength spectroscopy
* Forces in liquids
* unmarried molecule strength spectroscopy
* excessive answer imaging of organic material
* Imaging of force-distance curves
* excessive velocity AFM for looking at dynamic processes
* thought and fundamentals
* mix of AFM with optical methods
* organic purposes
* Electrochemical AFM
* Manipulation and lithography
An optimal stability for chemists, physicists, fabrics scientists, and biologists, in addition to analytical and medicinal chemists.

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M. (1998) Jumping mode scanning force microscopy. Appl. Phys. , 73, 3300–3302. , and Marti, O. (1997) The simultaneous measurement of elastic, electrostatic and adhesive properties by scanning force microscopy: pulsed-force mode operation. Meas. Sci. , 8, 1333–1338. M. (2002) Scanning force microscopy jumping and tapping modes in liquids. Appl. Phys. , 81, 2620–2623. J. (2006) DNA-mediated anisotropic mechanical reinforcement of a virus. Proc. Natl. Acad. Sci. , 103, 13706–13711. F. (2006) Elastic response, buckling, and instability of microtubules under radial indentation.

3 Approaching (a) and withdrawing (b) force-extension curves for a mica surface immersed in different buffers. 5) and 2 mM MgOAc supplemented with KCl or extra MgOAc when stated. 5 nN, a rather large force when imaging biological materials. 5), 50 mM KCl, 50 mM MgOAc (dark yellow curve) shows an effective absence of adhesion and attractive force. 3 Cantilever Dynamics in Liquids According to Eq. 1), the spring constant k only depends on the material properties of the cantilever and its geometrical dimensions.

First, as a consequence of the large density of the surrounding liquid compared with the density of air, the cantilever suffers an increase of the effective mass by a factor of 10–40 and a corresponding decrease of the resonant frequency (Eq. 3)). Resonance and natural frequencies are related by Eq. 8). Therefore as a second consequence, the strong hydrodynamic interaction between the cantilever and the liquid produces a very low quality factor Q. 5) [16]. The decrease in resonant frequency and Q has important consequences on the cantilever oscillation and therefore affects the performance of dynamic modes.

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